FUNCTIONAL CHARACTERIZATION OF CROP GENES ASSOCIATED WITH GROWTH AND STRESS RESPONSE
DOI:
https://doi.org/10.53555/gafs.v12i2.2580Keywords:
chilling stress, gene expression, functional characterization, rice, transcriptomicsAbstract
Cold stress influences plant growth and induces complex transcriptional mechanisms depending on the genetic background and the length of the exposure. We analyzed the genome-wide expression responses of chilling in three rice genotypes, NIP, CSSL4-1, and 93-11, using an FPKM-based transcriptomic dataset including 37,831 genes. We compared gene expression profiles between control and chilling conditions at 10 h and 41 h and performed fold change analysis, correlation assessment, principal component analysis, clustering, and functional characterization. Significant transcriptional reprogramming was observed in all genotypes, with CSSL4-1 having the most pronounced early induction. The shared response increased after longer exposure with 1,541 common up-regulated and 1,834 common down-regulated genes at 41 h . Integrated analysis also identified 237 consistently induced and 185 consistently repressed genes across all genotype-time comparisons. Functional characterization of responsive genes indicated their association with stress regulation, signaling, metabolism, redox homeostasis and growth-related processes. These results reveal genotype- and time-specific mechanisms of chilling adaptation and candidate genes for functional validation and stress-resilient rice improvement.
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